Anzalone, A. V. et al. Search-and-replace genome enhancing with out double-strand breaks or donor DNA. Nature 576, 149–157 (2019).
Google Scholar
Lin, Q. et al. Excessive-efficiency prime enhancing with optimized, paired pegRNAs in crops. Nat. Biotechnol. 39, 923–927 (2021).
Google Scholar
Zhuang, Y. et al. Growing the effectivity and precision of prime enhancing with information RNA pairs. Nat. Chem. Biol. 18, 29–37 (2022).
Google Scholar
Wang, J. et al. Environment friendly focused insertion of huge DNA fragments with out DNA donors. Nat. Strategies 19, 331–340 (2022).
Google Scholar
Choi, J. et al. Exact genomic deletions utilizing paired prime enhancing. Nat. Biotechnol. 40, 218–226 (2022).
Google Scholar
Jiang, T., Zhang, X.-O., Weng, Z. & Xue, W. Deletion and substitute of lengthy genomic sequences utilizing prime enhancing. Nat. Biotechnol. 40, 227–234 (2022).
Google Scholar
Chen, P. J. & Liu, D. R. Prime enhancing for exact and extremely versatile genome manipulation. Nat. Rev. Genet. 24, 161–177 (2023).
Google Scholar
Liu, B. et al. A cut up prime editor with untethered reverse transcriptase and round RNA template. Nat. Biotechnol. 40, 1388–1393 (2022).
Google Scholar
Nelson, J. W. et al. Engineered pegRNAs enhance prime enhancing effectivity. Nat. Biotechnol. 40, 402–410 (2022).
Google Scholar
Liu, Y. et al. Enhancing prime enhancing by Csy4-mediated processing of pegRNA. Cell Res. 31, 1134–1136 (2021).
Google Scholar
Zhang, G. et al. Enhancement of prime enhancing by way of xrRNA motif-joined pegRNA. Nat. Commun. 13, 1856 (2022).
Google Scholar
Li, X. et al. Enhancing prime enhancing effectivity by modified pegRNA with RNA G-quadruplexes. J. Mol. Cell. Biol. 14, mjac022 (2022).
Google Scholar
Feng, Y. et al. Enhancing prime enhancing effectivity and adaptability with tethered and cut up pegRNAs. Protein Cell 14, 304–308 (2022).
Google Scholar
Ponnienselvan, Okay. et al. Lowering the inherent auto-inhibitory interplay throughout the pegRNA enhances prime enhancing effectivity. Nucleic Acids Res. 51, 6966–6980 (2023).
Google Scholar
Ellefson, J. W. et al. Artificial evolutionary origin of a proofreading reverse transcriptase. Science 352, 1590–1593 (2016).
Google Scholar
Skasko, M. et al. Mechanistic variations in RNA-dependent DNA polymerization and constancy between murine leukemia virus and HIV-1 reverse transcriptases. J. Biol. Chem. 280, 12190–12200 (2005).
Google Scholar
Mathews, C. Okay. Deoxyribonucleotide metabolism, mutagenesis and most cancers. Nat. Rev. Most cancers 15, 528–539 (2015).
Google Scholar
Wu, W.-J., Yang, W. & Tsai, M.-D. How DNA polymerases catalyse replication and restore with contrasting constancy. Nat. Rev. Chem. 1, 0068 (2017).
Google Scholar
Truniger, V., Lázaro, J. M. & Salas, M. Two positively charged residues of phi29 DNA polymerase, conserved in protein-primed DNA polymerases, are concerned in stabilisation of the incoming nucleotide. J. Mol. Biol. 335, 481–494 (2004).
Google Scholar
Halperin, S. O. et al. CRISPR-guided DNA polymerases allow diversification of all nucleotides in a tunable window. Nature 560, 248–252 (2018).
Google Scholar
Tou, C. J., Schaffer, D. V. & Dueber, J. E. Focused diversification within the S. cerevisiae genome with CRISPR-guided DNA polymerase I. ACS Synth. Biol. 9, 1911–1916 (2020).
Google Scholar
Grünewald, J. et al. Engineered CRISPR prime editors with compact, untethered reverse transcriptases. Nat. Biotechnol. 41, 337–343 (2022).
Google Scholar
Ohtsubo, Y., Sasaki, H., Nagata, Y. & Tsuda, M. Optimization of single strand DNA incorporation response by Moloney murine leukaemia virus reverse transcriptase. DNA Res. 25, 477–487 (2018).
Google Scholar
Petri, Okay. et al. CRISPR prime enhancing with ribonucleoprotein complexes in zebrafish and first human cells. Nat. Biotechnol. 40, 189–193 (2022).
Google Scholar
Wang, Q. et al. Broadening the attain and investigating the potential of prime editors via absolutely viral gene-deleted adenoviral vector supply. Nucleic Acids Res. 49, 11986–12001 (2021).
Google Scholar
Liu, P. et al. Improved prime editors allow pathogenic allele correction and most cancers modelling in grownup mice. Nat. Commun. 12, 2121 (2021).
Google Scholar
Esteban, J. A., Salas, M. & Blanco, L. Constancy of phi 29 DNA polymerase. Comparability between protein-primed initiation and DNA polymerization. J. Biol. Chem. 268, 2719–2726 (1993).
Google Scholar
Lieberman, Okay. R. et al. Processive replication of single DNA molecules in a nanopore catalyzed by phi29 DNA polymerase. J. Am. Chem. Soc. 132, 17961–17972 (2010).
Google Scholar
Anzalone, A. V. et al. Programmable deletion, substitute, integration and inversion of huge DNA sequences with twin prime enhancing. Nat. Biotechnol. 40, 731–740 (2021).
Google Scholar
Blanco, L. et al. Extremely environment friendly DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication. J. Biol. Chem. 264, 8935–8940 (1989).
Google Scholar
Gillmore, J. D. et al. CRISPR–Cas9 in vivo gene enhancing for transthyretin amyloidosis. N. Engl. J. Med. 385, 493–502 (2021).
Google Scholar
Lee, R. G. et al. Efficacy and security of an investigational single-course CRISPR base-editing remedy focusing on PCSK9 in nonhuman primate and mouse fashions. Circulation 147, 242–253 (2023).
Google Scholar
Hopfner, Okay.-P. & Hornung, V. Molecular mechanisms and mobile features of cGAS–STING signalling. Nat. Rev. Mol. Cell Biol. 21, 501–521 (2020).
Google Scholar
Jiang, T. et al. Chemical modifications of adenine base editor mRNA and information RNA broaden its utility scope. Nat. Commun. 11, 1979 (2020).
Google Scholar
Zheng, C. et al. A versatile cut up prime editor utilizing truncated reverse transcriptase improves dual-AAV supply in mouse liver. Mol. Ther. 30, 1343–1351 (2022).
Google Scholar
Mir, A. et al. Closely and absolutely modified RNAs information environment friendly SpyCas9-mediated genome enhancing. Nat. Commun. 9, 2641 (2018).
Google Scholar
Liu, B. et al. Focused genome enhancing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates. NCBI Bioproject (2023).
