Nature Biotechnology, Printed on-line: 18 September 2023; doi:10.1038/s41587-023-01976-5
Through the use of mounted costs to engineer a robust electroosmotic movement, we obtain the unidirectional transport of pure polypeptides throughout nanopores. Our strategy permits native proteins to be transported enzymatically and non-enzymatically within the absence of denaturant and electrophoretic tags, with potential functions for protein sequencing.
